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metal ion affinity chromatography  (TaKaRa)


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    Structured Review

    TaKaRa metal ion affinity chromatography
    Metal Ion Affinity Chromatography, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 5148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/metal+ion+affinity+chromatography/TALON+Metal+Affinity+Resin/pm41720803-246-8-15
    Average 99 stars, based on 5148 article reviews
    metal ion affinity chromatography - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Affinity Chromatography:

    Article Title: Beta-arrestin 1 mediated Src activation via Src SH3 domain revealed by cryo-electron microscopy
    Article Snippet: .. SH3–βarr1 was separated from unbound βarr1 by immobilized metal ion affinity chromatography using Talon resin (Takara Bio). ..

    Article Title: Cell Surface Xyloglucan Recognition and Hydrolysis by the Human Gut Commensal Bacteroides uniformis.
    Article Snippet: .. Cells were harvested by centrifugation, and the His6-tagged protein was purified by immobilized metal ion affinity chromatography using cobalt-based Talon resin (TaKaRa Bio), with bound proteins eluted with 100 mM imidazole. .. Prior to crystallization experiments, BuXyGUL2 SGBP-B was further purified by size exclusion chromatography using Superdex 75 resin (GE Life Sciences) in 20 mM HEPES, pH 7.4, and 100 mM NaCl.

    Article Title: RAP1 regulates TIP60 function during fate transition between 2 cell-like and pluripotent states
    Article Snippet: Sonicated cell extracts were cleared by centrifugation and subsequently filtered (0.45 μm SterivexTM filter, Millipore). .. The supernatant containing Rap1 was further purified by Immobilized Metal ion Affinity Chromatography using TALON Metal Affinity Resin (Clontech) containing Co2+ cations as described ( ). ..

    Article Title: Mechanism of beta-arrestin 1 mediated Src activation via Src SH3 domain revealed by cryo-electron microscopy.
    Article Snippet: ITC measurements were performed using the MicroCal PEAQ-ITC system (Malvern). .. SH3–βarr1 was separated from unbound βarr1 by immobilized metal ion affinity chromatography using Talon resin (Takara Bio). ..

    Article Title: Structural and functional insights of the catalytic GH5 and Calx-β domains from the metagenome-derived endoglucanase CelE2.
    Article Snippet: Cellulose is the most abundant natural polymer on Earth, representing an attractive feedstock for bioproducts and biofuel production.. Cellulases promote the depolymerization of cellulose, generating short oligosaccharides and glucose, which are useful in biotechnological applications.. Among the classical cellulases, those from glycoside hydrolase family 5 (GH5) are one of the most abundant in Nature, displaying several modular architectures with other accessory domains attached to its catalytic core, such as carbohydrate-binding modules (CBMs), Ig-like, FN3-like, and Calx-β domains, which can influence the enzyme activity.

    Article Title: Prominent members of the human gut microbiota express endo-acting O-glycanases to initiate mucin breakdown
    Article Snippet: .. Cells were harvested, lysed by sonication, and the His-tagged protein was purified from the lysate by immobilised metal ion affinity chromatography using Talon resin (Clontech). ..

    Article Title: Cell Surface Xyloglucan Recognition and Hydrolysis by the Human Gut Commensal Bacteroides uniformis
    Article Snippet: .. Cells were harvested by centrifugation, and the His6-tagged protein was purified by immobilized metal ion affinity chromatography using cobalt-based Talon resin (TaKaRa Bio), with bound proteins eluted with 100 mM imidazole. .. Prior to crystallization experiments, BuXyGUL2 SGBP-B was further purified by size exclusion chromatography using Superdex 75 resin (GE Life Sciences) in 20 mM HEPES, pH 7.4, and 100 mM NaCl.

    Article Title: Rap1 regulates TIP60 function during fate transition between two-cell-like and pluripotent states.
    Article Snippet: Sonicated cell extracts were cleared by centrifugation and subsequently filtered (0.45-μm SterivexTM filter, Millipore). .. The supernatant containing Rap1 was further purified by immobilized metal ion affinity chromatography using TALON metal affinity resin (Clontech) containing Co2 + cations as described (Yanez et al. 2005). ..

    Centrifugation:

    Article Title: Cell Surface Xyloglucan Recognition and Hydrolysis by the Human Gut Commensal Bacteroides uniformis.
    Article Snippet: .. Cells were harvested by centrifugation, and the His6-tagged protein was purified by immobilized metal ion affinity chromatography using cobalt-based Talon resin (TaKaRa Bio), with bound proteins eluted with 100 mM imidazole. .. Prior to crystallization experiments, BuXyGUL2 SGBP-B was further purified by size exclusion chromatography using Superdex 75 resin (GE Life Sciences) in 20 mM HEPES, pH 7.4, and 100 mM NaCl.

    Article Title: Cell Surface Xyloglucan Recognition and Hydrolysis by the Human Gut Commensal Bacteroides uniformis
    Article Snippet: .. Cells were harvested by centrifugation, and the His6-tagged protein was purified by immobilized metal ion affinity chromatography using cobalt-based Talon resin (TaKaRa Bio), with bound proteins eluted with 100 mM imidazole. .. Prior to crystallization experiments, BuXyGUL2 SGBP-B was further purified by size exclusion chromatography using Superdex 75 resin (GE Life Sciences) in 20 mM HEPES, pH 7.4, and 100 mM NaCl.

    Purification:

    Article Title: Cell Surface Xyloglucan Recognition and Hydrolysis by the Human Gut Commensal Bacteroides uniformis.
    Article Snippet: .. Cells were harvested by centrifugation, and the His6-tagged protein was purified by immobilized metal ion affinity chromatography using cobalt-based Talon resin (TaKaRa Bio), with bound proteins eluted with 100 mM imidazole. .. Prior to crystallization experiments, BuXyGUL2 SGBP-B was further purified by size exclusion chromatography using Superdex 75 resin (GE Life Sciences) in 20 mM HEPES, pH 7.4, and 100 mM NaCl.

    Article Title: RAP1 regulates TIP60 function during fate transition between 2 cell-like and pluripotent states
    Article Snippet: Sonicated cell extracts were cleared by centrifugation and subsequently filtered (0.45 μm SterivexTM filter, Millipore). .. The supernatant containing Rap1 was further purified by Immobilized Metal ion Affinity Chromatography using TALON Metal Affinity Resin (Clontech) containing Co2+ cations as described ( ). ..

    Article Title: Structural and functional insights of the catalytic GH5 and Calx-β domains from the metagenome-derived endoglucanase CelE2.
    Article Snippet: Cellulose is the most abundant natural polymer on Earth, representing an attractive feedstock for bioproducts and biofuel production.. Cellulases promote the depolymerization of cellulose, generating short oligosaccharides and glucose, which are useful in biotechnological applications.. Among the classical cellulases, those from glycoside hydrolase family 5 (GH5) are one of the most abundant in Nature, displaying several modular architectures with other accessory domains attached to its catalytic core, such as carbohydrate-binding modules (CBMs), Ig-like, FN3-like, and Calx-β domains, which can influence the enzyme activity.

    Article Title: Prominent members of the human gut microbiota express endo-acting O-glycanases to initiate mucin breakdown
    Article Snippet: .. Cells were harvested, lysed by sonication, and the His-tagged protein was purified from the lysate by immobilised metal ion affinity chromatography using Talon resin (Clontech). ..

    Article Title: Cell Surface Xyloglucan Recognition and Hydrolysis by the Human Gut Commensal Bacteroides uniformis
    Article Snippet: .. Cells were harvested by centrifugation, and the His6-tagged protein was purified by immobilized metal ion affinity chromatography using cobalt-based Talon resin (TaKaRa Bio), with bound proteins eluted with 100 mM imidazole. .. Prior to crystallization experiments, BuXyGUL2 SGBP-B was further purified by size exclusion chromatography using Superdex 75 resin (GE Life Sciences) in 20 mM HEPES, pH 7.4, and 100 mM NaCl.

    Article Title: Rap1 regulates TIP60 function during fate transition between two-cell-like and pluripotent states.
    Article Snippet: Sonicated cell extracts were cleared by centrifugation and subsequently filtered (0.45-μm SterivexTM filter, Millipore). .. The supernatant containing Rap1 was further purified by immobilized metal ion affinity chromatography using TALON metal affinity resin (Clontech) containing Co2 + cations as described (Yanez et al. 2005). ..

    Sonication:

    Article Title: Prominent members of the human gut microbiota express endo-acting O-glycanases to initiate mucin breakdown
    Article Snippet: .. Cells were harvested, lysed by sonication, and the His-tagged protein was purified from the lysate by immobilised metal ion affinity chromatography using Talon resin (Clontech). ..



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